The Quantitative Proteomics Core at the Cancer Science Institute of Singapore provides comprehensive support for proteomics studies, from experimental design and sample preparation through mass spectrometry analysis, bioinformatics and data interpretation. We work closely with researchers across a broad range of applications, including quantitative proteome profiling, interactomics, imaging mass spectrometry and the analysis of clinically relevant samples.
We currently operate a Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer as well as a timsTOF flex in combination with an EASY-nLC 1200 uHPLC system or an Evosep ONE. Homogenisation of clinical tissues is performed with a BeatBox tissue homogeniser. The data analysis is performed on in-house servers using the freely available quantitative proteomics software packages MaxQuant and DIA-NN. We are committed to providing quantitative mass spectrometry solutions and to support researchers in using label-free quantitation (LFQ).
Facility Head
Dr. Dennis KAPPEI
Dennis’ research group uses their expertise in quantitative proteomics to study chromatin changes in the context of telomere-driven genome instability. Through the Quantitative Proteomics Core, this know-how in mass spectrometry applications is shared with CSI and the wider Singapore research community.
Team
Dr Wai Khang YONG
Core Facility Manager
Wai Khang earned a PhD from NUS Graduate School's Integrative Sciences and Engineering Programme (ISEP). His research centers on investigating chromatin protein-DNA interactions through ChIP-MS. With meticulous attention to technical nuances, he has implemented in-house experimental pipelines for ChIP-MS, surface membrane proteomics, and PTM enrichment.
Dr Sakshi RAJORIA
Research Fellow
After graduate training at the Defence Research and Development Establishment in Madhya Pradesh, Sakshi spent initial postdoctoral stints at IIT Bombay and the University of Massachusetts. In our team she is developing spatial proteomics workflows to analyse tissue samples by imaging mass spectrometry.
Vartika KHANCHANDANI
Research Associate
Vartika earned a Bachelor of Engineering from the R.V. College of Engineering in Bangalore. In our group, she is applying her bioinformatics skills to the development of streamlined analysis pipelines.
Celine Eleora WIHARJA
Research Assistant
Celine obtained her Bachelor of Biomedical Science from the Indonesia International Institute of Life Sciences. As part of the QPC, she supports sample preparation, mass spectrometry workflows, and data processing across a range of proteomics applications and diverse biological contexts.