The Facility currently operates two Becton-Dickinson FACSAria machines for cell sorting and analysis. These instruments are equipped with 3 or 5 air-cooled lasers and can perform 9 to 12 color flow cytometry analyses. They support 2 and 4-way bulk sorting to tubes, as well as single-cell sorting to multi-well plates (ranging from 6 to 384 wells) or microscope slides.

Additionally, we have a Becton-Dickinson FACSMelody sorter that is capable of up to 8 color sorting. It also supports 2 and 4-way bulk sorting to tubes and single-cell sorting to well plates (ranging from 6 to 384 wells). The FACSMelody features user-friendly FACSChorus software, allowing for self-operation. All three sorters can operate under aseptic conditions, making the sorted cells suitable for culture.

The Facility also runs a high throughput flow analyzer, the Becton-Dickinson FACS Celesta. This machine is equipped with 3 lasers and 13 detectors, making it ideal for drug screening and immunoprofiling applications.

To meet the growing demand for high-parameter data analyses, such as immunophenotyping, intracellular molecule quantification, cell activation, cell proliferation, cell cycle analysis, apoptosis, and measurement of cellular metabolism, we have recently acquired a Becton-Dickinson FACSymphony A3 with 5 lasers. This advanced instrument enables users to perform up to 28-color analyses.