NUS CSI

CSI-Advances in Research (AIR) Meeting
2019-04-16 07:07 UTC

16th Asia-Pacific International Molecular Biology Network Conference
2019-04-16 07:07

Joint SGCC-CSI Gastric Cancer Seminar
2019-04-16 07:07

CSI RESEARCH MEETING
2019-04-16 07:07

CSI-Advances in Research – YONG Kol Jia
2019-04-16 07:07

Frontiers in Cancer Science 2015
2019-04-16 07:07

CSI Research Meeting - 16 Jun
2019-04-16 07:07

NUS Scientists Develop Novel Chemical "Dye" to Improve Liver Cancer Imaging
2019-04-16 07:07

NUS-CMU Joint Symposium
2019-04-16 07:07

RNA Club – 9 OCT
2019-04-16 07:07

SRBA Meeting - 21 Feb
2019-04-16 07:07

CSI Seminar - Li Chai
2019-04-16 07:07

RNA Biology Centre Meeting - 23 Apr
2019-04-23 03:00 - 04:00
Venue: Level 12 Conference Room
Time: 11am
Scientific work presentation:
Speaker: Shi Hao TAN (from Takaomi Sanda’s group)
Title: Identification of Long Non-Coding RNAs Regulated by the TAL1 Complex in T-cell Acute Lymphoblastic Leukemia
Abstract:
TAL1/SCL is one of the most prevalent oncogenes in T-cell acute lymphoblastic leukemia (T-ALL). TAL1 and its regulatory partners (GATA3, RUNX1, and MYB) positively regulate each other and coordinately regulate the expression of their downstream target genes in T-ALL cells. However, long non-coding RNAs (lncRNAs) regulated by these factors are largely unknown. Here we established a bioinformatics pipeline and analyzed RNA-seq datasets to identify lncRNAs regulated by TAL1 in T-ALL. Our analysis predicted 57 putative lncRNAs that are activated by TAL1, many of which were regulated by GATA3, RUNX1, and MYB in a coordinated manner. We identified two novel transcripts that were activated in multiple T-ALL cell samples but were downregulated in normal thymocytes. One novel lncRNA transcript near the ARID5B gene locus which we named ARIEL (ARID5B Inducing Enhancer-associated Long Non-Coding RNA) was specifically expressed in TAL1-positive T-ALL cases. ARID5B was recently identified as important for T-ALL leukemogenesis and we further explored the role of the neighbourhood lncRNA ARIEL. Further analysis our Chip-seq data showed that ARIEL is aberrantly expressed from the -135kb enhancer region of ARID5B in TAL1-positive cells. Knockdown of ARIEL resulted in a significant decrease of the levels of ARID5B at the mRNA and protein levels together with a loss of chromatin interaction between this enhancer and ARID5B promoter region as observed by 4C-seq. We also show through RNA Immunoprecipitation (RIP) that ARIEL is able to interact with Mediator 12. Overall, our study shows that the ARIEL can act as an enhancer RNA to regulate expression of the oncogenic ARID5B by mediating enhancer-promoter chromatin interaction in T-ALL leukemogenesis.

CSI Research Meeting - 26 April
2019-04-26 05:00 - 06:00

RNA Club - 29 Apr
2019-04-29 03:00 - 04:00
Speaker: Assoc Prof. LU JinhuaVenue: NUS, MD6, Level 12 conference room
Time: 11 am
Title: Ribosome biogenesis and regulation
Host: Dr Polly Chen

CSI Seminar - Claudio Tripodo
2019-05-03 03:00

CSI Seminar - Chen Jianjun
2019-05-08 07:00 - 08:00

CSI Research Meeting - 10 May
2019-05-10 05:00 - 06:00

RNA Biology Centre Meeting – 14 May
2019-05-14 03:00 - 04:00
Scientific work presentation:Speaker: Huang Hua (from Soong Tuck Wah’s group)
1st short talk title: Evolutionary conservation and physiological roles of A-to-I RNA editing in CaV1.3 channels
Abstract:
We have reported that voltage gated calcium channel CaV1.3 undergoes ADAR2 mediated A-to-I RNA editing in a developmental and tissue selective fashion. Our recent paper demonstrated such editing is dictated by an intronic editing complementary sequence (ECS) that forms RNA duplex structure with the edited exon 41. Intriguingly, in silico alignment in the encode database demonstrated high conservation of ECS and exonic sequence across vertebrates. Correlatingly, editing of CaV1.3 mRNA was observed in most of experimental animal models such as rodents, pig, zebra fishes and macaque. Further tissues profiling of zebra fishes reports lack of editing in peripheral tissues such as muscle and testis, a trend similarly observed in rodents and human. Behaviour study showed mice lack of editing of Cav1.3 displayed longer retention of fear memory. We have similarly deleted the ECS sequence in zebra fish. Experiments are underway to understand the physiological roles of such molecular event in evolution.
2nd short talk title: Identification of tandem pore domain potassium channel KCNK5 as a novel target in Ph+ CML
Abstract:
Potassium channels are pore-forming transmembrane proteins that regulate a variety of cellular processes via controlling the potassium flow across biological membrane. Accumulating data implicates the dysregulation of potassium channel expression in different types of cancer. In the present study, quantitative real-time PCR reported overexpression of KCNK5 mRNA in K562 cells and Ph+ CML bone marrow samples as compared to normal bone marrow. Moreover, the expression of the KCNK5 mRNA and protein in K562 cells are sensitive to imatinib that blocks BCR-ABL tyrosine-kinase activity. In silico analysis of the Chip-seq database revealed strong interaction of c-MYC and Max binding to the promoter region of KCNK5, suggesting that BCR-ABL induced ectopic expression of the channel via Jak2 and c-MYC pathway. Sub-cellular fractionation experiment detects enrichment of KCNK5 channels in the lysosomal fraction, suggesting the potential function of the channels in regulating cellular metabolism. KCNK5 specific blocker clofilium inhibits channel function and proliferation of K562 cells. CRISPR/Cas9 mediated gene knockout (KO) in K562 cells suppresses tumor formation in mouse xenograft model. By tail vein injection, mice engrafted with KO cells survived significantly longer than mice transplanted with control cells. Taken together the study reports identification of KCNK5 potentially as a novel target in Ph+ CML.

CSI Research Meeting - 24 May
2019-05-24 05:00 - 06:00

Bioinformatics Club
2019-05-27 06:00 - 09:00
Title: Increase efficiency of OMICs data analysis and interpretation with MetaCoreSpeaker: Sonya Novikova, Solutions Scientist from Clarivate Analytics
Abstract:
MetaCore is a systems biology database with in-build tools and workflows for OMICs data analysis and biological interpretation. Data contained within MetaCore is industry leading in accuracy, reliability, and comprehensiveness, manually curated by PhD and MD level research professionals and updated quarterly. Key Pathway Advisor (KPA), a MetaCore’s complementary solution, enhances MetaCore’s data analysis workflows by using MetaCore’s content and state-of-the-art algorithms, causal reasoning and Signaling Pathway Impact Anlaysis (SPIA), to predict key protein activity changes that could be the root cause for the changes observed in your data and shows how biological pathways are impacted by your data. Integrate your experimental research with manually-curated molecular interactions and pathways for pivotal insights and decision making, particularly in

Identifying signaling pathways and processes affected by your gene lists or expression data Understand molecular mechanisms of disease and/or drug action
Overlaying and visualize your gene lists or expression data on pathways and networks Enhance results interpretation and accuracy – find drug targets and/or biomarkers
Discovering key hubs overconnected to your genes (e.g. key transcription factors that potentially drive observed gene expression changes) Look beyond your gene list/ expression data – use network of interaction to find “hidden nodes”
Connecting your molecules of interest to reveal hidden relationships Understand molecular mechanisms of disease and/or drug action

All are welcome. Bring your gene lists which you want to find out the pathways or interaction networks. The online access to the pathway/network analysis suites will be provided. However, you will need to bring your own laptop for online trial analyses; Tea/Coffee and few snacks will be provided
Venue: Conference room at Level 12, MD6
Time: May 27, 2019, 2.00-5.00pm (including 2+hr for online trial analyses with your own gene sets)

RNA Biology Centre Meeting - 28 May
2019-05-28 03:00 - 04:00
To be updated

CSI Seminar - Daniel Hodson
2019-05-28 06:00 - 07:00

CSI Seminar - Joe Yeong
2019-06-06 03:00 - 04:00

CSI Research Meeting & Industry Liaison Office Presentation - 7 June
2019-06-07 05:00 - 06:00

CSI Seminar - Michael Beer
2019-06-12 03:00 - 04:00

CSI Research Meeting - 21 June
2019-06-21 05:00 - 06:00

3rd Peritoneal Surface Malignancy Conference
2019-06-27 00:00 - 2019-06-28 09:00
For enquiries, contact the secretariat at division.of.surgical.oncology@nccs.com.sg

Meet-The-Authors Seminar - Dept of Physiology, NUS
2019-06-28 04:00 - 05:00

CSI Research Meeting - 5 Jul
2019-07-05 05:00 - 06:00
