NUS CSI

CSI Seminar - Chiang Cheng-Ming
2019-01-18 06:30 - 07:30 UTC

CSI Distinguished Speakers’ Series – Samuel Waxman
2019-01-21 06:30 - 07:30

CSI Research Meeting - 25 Jan
2019-01-25 05:00 - 06:00

CSI Distinguished Speakers’ Series – Reuben Harris
2019-02-18 00:00 - 09:00

SRBA Meeting - 21 Feb
2019-02-21 08:00 - 09:30, 2019-04-16 07:07

CSI Research Meeting - 22 Feb
2019-02-22 05:00 - 06:00

RNA Biology Centre Meeting - 26 Feb
2019-02-26 03:00 - 04:00
Venue: Level 12 Conference Room
Time: 11am
Scientific work presentation:
Speaker: Xin Yi LOH (Prof H. Phillip Koeffler’s group)
Title: ZFP36L1 suppresses hypoxia and cell cycle signaling
Abstract:
ZFP36L1 is a tandem zinc-finger RNA binding protein (RBP) that recognizes conserved Adenylate-Uridylate-rich elements (AU-rich elements, AREs) located in 3’untranslated regions (UTRs) mediating mRNAs decay process. Here, we hypothesized that ZFP36L1 is a negative regulator of a post-transcriptional hub involving in mRNA half-life regulation of cancer-related transcripts. To identify the direct downstream targets of ZFP36L1, a systematic screening using RNA pull-down and whole transcriptome sequencing of bladder cancer cells ± ZFP36L1 was performed. Analysis of in silico data revealed that ZFP36L1 is significantly mutated, epigenetically silenced and downregulated in a variety of cancers. Forced expression of ZFP36L1 in cancer cells markedly reduced in vitro and in vivo cell proliferation; whereas silencing of ZFP36L1 enhanced tumor cells growth. We identified a network of 1,410 targets, which were bound by wildtype, but not zinc finger mutant ZFP36L1. These targets included a number of key oncogenic transcripts, such as HIF-1?, CCND1 and E2F1. ZFP36L1 specifically bound to 3’UTRs of these targets for mRNA degradation, thus suppressing their mRNA and protein expressions. Dual luciferase reporter assays and RNA Electro-Mobility Shift Assays (RNA-EMSA) showed that wildtype, but not zinc finger mutant ZFP36L1, bound to HIF-1? 3’UTR and mediated HIF-1? mRNA degradation. This eventually leads to reduced expressions of HIF-1? and its downstream targets in metabolism and angiogenesis. Collectively, our findings shed light on the pathophysiology of bladder tumorigenesis and revealed an indispensable role of ZFP36L1 as a posttranscriptional safeguard against aberrant hypoxic signaling and abnormal cell cycle progression.

CSI Seminar - Peter Kharchenko
2019-02-27 06:30 - 07:30

RNA Club – 4 Mar
2019-03-04 03:00 - 04:00

CSI Seminar - Simón Méndez-Ferrer
2019-03-05 08:00 - 09:00

[CANCELLED] CSI Seminar - Yan Chuan
2019-03-07 03:00 - 04:00
Due to unforeseen circumstances, this seminar is CANCELLED.

CSI Research Meeting - 8 March
2019-03-08 05:00 - 06:00

CSI Seminar - Yoshiaki Kubota
2019-03-08 08:00 - 09:00

CSI Seminar - Ichiro Taniuchi
2019-03-21 03:00 - 04:00

CSI Research Meeting - 22 March
2019-03-22 05:00 - 06:00

CSI Research Meeting - 5 April
2019-04-05 05:00 - 06:00
The CSI State-of-the-Institute address 2019 will commence at 1pm.Regular CSI Research Meeting talks as below will start after the State-of-the-Institute address, followed by refreshments at 2.30pm.
All staff and students are encouraged to attend.

RNA Club - 8 Apr
2019-04-08 03:00 - 04:00
Speaker: Dr Jordan SkittrallVenue: NUS, MD6, Level 12 conference room
Time: 11 am
Title: In silico prediction of RNA secondary structure and function using big data approaches to primary structures|
Host: Prof. Andrew Lever

Bioinformatics Workshop - 16 Apr
2019-04-16 05:00 - 09:00

CSI-Advances in Research (AIR) Meeting
2019-04-16 07:07

16th Asia-Pacific International Molecular Biology Network Conference
2019-04-16 07:07

Joint SGCC-CSI Gastric Cancer Seminar
2019-04-16 07:07

CSI RESEARCH MEETING
2019-04-16 07:07

CSI-Advances in Research – YONG Kol Jia
2019-04-16 07:07

Frontiers in Cancer Science 2015
2019-04-16 07:07

CSI Research Meeting - 16 Jun
2019-04-16 07:07

NUS Scientists Develop Novel Chemical "Dye" to Improve Liver Cancer Imaging
2019-04-16 07:07

NUS-CMU Joint Symposium
2019-04-16 07:07

RNA Club – 9 OCT
2019-04-16 07:07

CSI Seminar - Li Chai
2019-04-16 07:07
