NUS CSI

CSI-TBSI Joint Symposium on Precision Approaches in Cancer
2018-11-05 00:00 - 09:00 UTC

CSI-CityU Joint RNA Symposium
2018-11-06 01:00 - 10:30

Frontiers in Cancer Science 2018
2018-11-12 01:00 - 2018-11-14 10:00

CSI Seminar - Li Chai
2018-12-06 06:30 - 07:30

CSI Seminar - Hubert Hondermarck
2018-12-12 02:30 - 15:30

CSI Seminar - Tannishtha Reya
2018-12-21 02:30 - 15:30

RNA Biology Centre Meeting - 8 Jan
2019-01-08 03:00 - 04:00
By Invitation Only
Date: 8 Jan 2019, Tuesday
Time: 11am
Venue: MD6, Level 12 Conference Room (#12-02N)

Updates from:
– Prof. Dan Tenen on MOE Tier 3 grant
– Dr Melissa Fullwood on RNA Epigenomics Core – Personnel updates; LncRNA microarray updates

Scientific Presentation:
Speaker: Wei Wen TEO (Prof Daniel Tenen’s group)
Title: Non-coding RNA Mediates Long-range Regulation of ?-Globin by Inhibiting EZH2

Abstract:
The best established function of EZH2-RNA interaction is gene inactivation. Nevertheless, several lines of evidence indicates that repression might not be the only function of EZH2-RNA interaction .In this study, we investigate the functional role of EZH2-RNA interaction in K562 cells. RIP analysis reveals that EZH2 binds to a non-coding RNA (HBE-ER) transcribed upstream of ?-globin cluster. Depletion of HBE-ER in K562 cells enhances the methyl-transferase activity of EZH2 at the corresponding locus, suggesting a cis-acting role of HBE-ER at the genomic locus. HBE-ER transcript permits the distal chromatin interaction between its locus and the locus control region (LCR) of ?-globin. Paused expression of HBE-ER releases its locus engagement with the LCR, thereby allowing the enhancer to contact with HBE1 promoter. This results in the activation of HBE1 transcription, which is evidenced by the enhanced Pol2 binding at its promoter. The transcription of HBE-ER is negatively regulated by oxygen depletion. In contrast, HBE1 level increases under hypoxia, suggesting an oxygen sensing role for controlling the expression of HBE1 by HBE-ER.

CSI Seminar - Constanze Bonifer & Peter Cockerill
2019-01-11 00:00 - 09:00

CSI Seminar - Charles David
2019-01-18 02:00 - 03:00

CSI Seminar - Chiang Cheng-Ming
2019-01-18 06:30 - 07:30

CSI Distinguished Speakers’ Series – Samuel Waxman
2019-01-21 06:30 - 07:30

CSI Research Meeting - 25 Jan
2019-01-25 05:00 - 06:00

CSI Distinguished Speakers’ Series – Reuben Harris
2019-02-18 00:00 - 09:00

SRBA Meeting - 21 Feb
2019-02-21 08:00 - 09:30

CSI Research Meeting - 22 Feb
2019-02-22 05:00 - 06:00

RNA Biology Centre Meeting - 26 Feb
2019-02-26 03:00 - 04:00
Venue: Level 12 Conference Room
Time: 11am
Scientific work presentation:
Speaker: Xin Yi LOH (Prof H. Phillip Koeffler’s group)
Title: ZFP36L1 suppresses hypoxia and cell cycle signaling
Abstract:
ZFP36L1 is a tandem zinc-finger RNA binding protein (RBP) that recognizes conserved Adenylate-Uridylate-rich elements (AU-rich elements, AREs) located in 3’untranslated regions (UTRs) mediating mRNAs decay process. Here, we hypothesized that ZFP36L1 is a negative regulator of a post-transcriptional hub involving in mRNA half-life regulation of cancer-related transcripts. To identify the direct downstream targets of ZFP36L1, a systematic screening using RNA pull-down and whole transcriptome sequencing of bladder cancer cells ± ZFP36L1 was performed. Analysis of in silico data revealed that ZFP36L1 is significantly mutated, epigenetically silenced and downregulated in a variety of cancers. Forced expression of ZFP36L1 in cancer cells markedly reduced in vitro and in vivo cell proliferation; whereas silencing of ZFP36L1 enhanced tumor cells growth. We identified a network of 1,410 targets, which were bound by wildtype, but not zinc finger mutant ZFP36L1. These targets included a number of key oncogenic transcripts, such as HIF-1?, CCND1 and E2F1. ZFP36L1 specifically bound to 3’UTRs of these targets for mRNA degradation, thus suppressing their mRNA and protein expressions. Dual luciferase reporter assays and RNA Electro-Mobility Shift Assays (RNA-EMSA) showed that wildtype, but not zinc finger mutant ZFP36L1, bound to HIF-1? 3’UTR and mediated HIF-1? mRNA degradation. This eventually leads to reduced expressions of HIF-1? and its downstream targets in metabolism and angiogenesis. Collectively, our findings shed light on the pathophysiology of bladder tumorigenesis and revealed an indispensable role of ZFP36L1 as a posttranscriptional safeguard against aberrant hypoxic signaling and abnormal cell cycle progression.

CSI Seminar - Peter Kharchenko
2019-02-27 06:30 - 07:30

RNA Club – 4 Mar
2019-03-04 03:00 - 04:00

CSI Seminar - Simón Méndez-Ferrer
2019-03-05 08:00 - 09:00

[CANCELLED] CSI Seminar - Yan Chuan
2019-03-07 03:00 - 04:00
Due to unforeseen circumstances, this seminar is CANCELLED.

CSI Research Meeting - 8 March
2019-03-08 05:00 - 06:00

CSI Seminar - Yoshiaki Kubota
2019-03-08 08:00 - 09:00

CSI Seminar - Ichiro Taniuchi
2019-03-21 03:00 - 04:00

CSI Research Meeting - 22 March
2019-03-22 05:00 - 06:00

CSI Research Meeting - 5 April
2019-04-05 05:00 - 06:00
The CSI State-of-the-Institute address 2019 will commence at 1pm.Regular CSI Research Meeting talks as below will start after the State-of-the-Institute address, followed by refreshments at 2.30pm.
All staff and students are encouraged to attend.

RNA Club - 8 Apr
2019-04-08 03:00 - 04:00
Speaker: Dr Jordan SkittrallVenue: NUS, MD6, Level 12 conference room
Time: 11 am
Title: In silico prediction of RNA secondary structure and function using big data approaches to primary structures|
Host: Prof. Andrew Lever

Bioinformatics Workshop - 16 Apr
2019-04-16 05:00 - 09:00

CSI-Advances in Research (AIR) Meeting
2019-04-16 07:07

16th Asia-Pacific International Molecular Biology Network Conference
2019-04-16 07:07

Joint SGCC-CSI Gastric Cancer Seminar
2019-04-16 07:07
