CSI Research Meeting - 25 Jan
2019-01-25 05:00 - 06:00 UTC

CSI Distinguished Speakers’ Series – Reuben Harris
2019-02-18 00:00 - 09:00

SRBA Meeting - 21 Feb
2019-02-21 08:00 - 09:30, 2019-04-16 07:07

CSI Research Meeting - 22 Feb
2019-02-22 05:00 - 06:00

RNA Biology Centre Meeting - 26 Feb
2019-02-26 03:00 - 04:00
Venue: Level 12 Conference Room
Time: 11am
Scientific work presentation:
Speaker: Xin Yi LOH (Prof H. Phillip Koeffler’s group)
Title: ZFP36L1 suppresses hypoxia and cell cycle signaling
Abstract:
ZFP36L1 is a tandem zinc-finger RNA binding protein (RBP) that recognizes conserved Adenylate-Uridylate-rich elements (AU-rich elements, AREs) located in 3’untranslated regions (UTRs) mediating mRNAs decay process. Here, we hypothesized that ZFP36L1 is a negative regulator of a post-transcriptional hub involving in mRNA half-life regulation of cancer-related transcripts. To identify the direct downstream targets of ZFP36L1, a systematic screening using RNA pull-down and whole transcriptome sequencing of bladder cancer cells ± ZFP36L1 was performed. Analysis of in silico data revealed that ZFP36L1 is significantly mutated, epigenetically silenced and downregulated in a variety of cancers. Forced expression of ZFP36L1 in cancer cells markedly reduced in vitro and in vivo cell proliferation; whereas silencing of ZFP36L1 enhanced tumor cells growth. We identified a network of 1,410 targets, which were bound by wildtype, but not zinc finger mutant ZFP36L1. These targets included a number of key oncogenic transcripts, such as HIF-1?, CCND1 and E2F1. ZFP36L1 specifically bound to 3’UTRs of these targets for mRNA degradation, thus suppressing their mRNA and protein expressions. Dual luciferase reporter assays and RNA Electro-Mobility Shift Assays (RNA-EMSA) showed that wildtype, but not zinc finger mutant ZFP36L1, bound to HIF-1? 3’UTR and mediated HIF-1? mRNA degradation. This eventually leads to reduced expressions of HIF-1? and its downstream targets in metabolism and angiogenesis. Collectively, our findings shed light on the pathophysiology of bladder tumorigenesis and revealed an indispensable role of ZFP36L1 as a posttranscriptional safeguard against aberrant hypoxic signaling and abnormal cell cycle progression.

CSI Seminar - Peter Kharchenko
2019-02-27 06:30 - 07:30

RNA Club – 4 Mar
2019-03-04 03:00 - 04:00

CSI Seminar - Simón Méndez-Ferrer
2019-03-05 08:00 - 09:00

[CANCELLED] CSI Seminar - Yan Chuan
2019-03-07 03:00 - 04:00
Due to unforeseen circumstances, this seminar is CANCELLED.

CSI Research Meeting - 8 March
2019-03-08 05:00 - 06:00

CSI Seminar - Yoshiaki Kubota
2019-03-08 08:00 - 09:00

CSI Seminar - Ichiro Taniuchi
2019-03-21 03:00 - 04:00

CSI Research Meeting - 22 March
2019-03-22 05:00 - 06:00

CSI Research Meeting - 5 April
2019-04-05 05:00 - 06:00
The CSI State-of-the-Institute address 2019 will commence at 1pm.Regular CSI Research Meeting talks as below will start after the State-of-the-Institute address, followed by refreshments at 2.30pm.
All staff and students are encouraged to attend.

RNA Club - 8 Apr
2019-04-08 03:00 - 04:00
Speaker: Dr Jordan SkittrallVenue: NUS, MD6, Level 12 conference room
Time: 11 am
Title: In silico prediction of RNA secondary structure and function using big data approaches to primary structures|
Host: Prof. Andrew Lever

Bioinformatics Workshop - 16 Apr
2019-04-16 05:00 - 09:00

CSI-Advances in Research (AIR) Meeting
2019-04-16 07:07

16th Asia-Pacific International Molecular Biology Network Conference
2019-04-16 07:07

Joint SGCC-CSI Gastric Cancer Seminar
2019-04-16 07:07

CSI-Advances in Research – YONG Kol Jia
2019-04-16 07:07

CSI RESEARCH MEETING
2019-04-16 07:07

Frontiers in Cancer Science 2015
2019-04-16 07:07

NUS Scientists Develop Novel Chemical "Dye" to Improve Liver Cancer Imaging
2019-04-16 07:07

CSI Research Meeting - 16 Jun
2019-04-16 07:07

NUS-CMU Joint Symposium
2019-04-16 07:07

CSI Seminar - Li Chai
2019-04-16 07:07

RNA Club – 9 OCT
2019-04-16 07:07

RNA Biology Centre Meeting - 23 Apr
2019-04-23 03:00 - 04:00
Venue: Level 12 Conference Room
Time: 11am
Scientific work presentation:
Speaker: Shi Hao TAN (from Takaomi Sanda’s group)
Title: Identification of Long Non-Coding RNAs Regulated by the TAL1 Complex in T-cell Acute Lymphoblastic Leukemia
Abstract:
TAL1/SCL is one of the most prevalent oncogenes in T-cell acute lymphoblastic leukemia (T-ALL). TAL1 and its regulatory partners (GATA3, RUNX1, and MYB) positively regulate each other and coordinately regulate the expression of their downstream target genes in T-ALL cells. However, long non-coding RNAs (lncRNAs) regulated by these factors are largely unknown. Here we established a bioinformatics pipeline and analyzed RNA-seq datasets to identify lncRNAs regulated by TAL1 in T-ALL. Our analysis predicted 57 putative lncRNAs that are activated by TAL1, many of which were regulated by GATA3, RUNX1, and MYB in a coordinated manner. We identified two novel transcripts that were activated in multiple T-ALL cell samples but were downregulated in normal thymocytes. One novel lncRNA transcript near the ARID5B gene locus which we named ARIEL (ARID5B Inducing Enhancer-associated Long Non-Coding RNA) was specifically expressed in TAL1-positive T-ALL cases. ARID5B was recently identified as important for T-ALL leukemogenesis and we further explored the role of the neighbourhood lncRNA ARIEL. Further analysis our Chip-seq data showed that ARIEL is aberrantly expressed from the -135kb enhancer region of ARID5B in TAL1-positive cells. Knockdown of ARIEL resulted in a significant decrease of the levels of ARID5B at the mRNA and protein levels together with a loss of chromatin interaction between this enhancer and ARID5B promoter region as observed by 4C-seq. We also show through RNA Immunoprecipitation (RIP) that ARIEL is able to interact with Mediator 12. Overall, our study shows that the ARIEL can act as an enhancer RNA to regulate expression of the oncogenic ARID5B by mediating enhancer-promoter chromatin interaction in T-ALL leukemogenesis.

CSI Research Meeting - 26 April
2019-04-26 05:00 - 06:00
